Your lab discovers a compound named X that they believe may be a tyrosine kinase inhibitor. What experiments would you perform to determine if X has the activity to block tumorigenesis in cell lines? (note: think of signaling pathways and the six hallmarks of cancer)
Unfortunately, preliminary experiments indicate X was not effective in the cancer type you chose to study. What might be some reasons that the inhibitor was not effective? How might you make use of this inhibitor (without changing its structure) to stop tumorigenesis even though it is currently not effective?